The Defender’s passenger compartment remained stable in the offset frontal test. Dummy readings of femur forces stiff structures in the dashboard and the score for protection of this body area was penalised. The vehicle’s high mass and front structure makes it an aggressive partner to a colliding vehicle and the score for the frontal offset test was reduced as a consequence. In the full-width, rigid wall test, protection was good or adequate for all body critical body regions, except for the chest of the rear passenger, protection of which was rated as marginal, based on readings of chest compression. In both the side barrier test, representing a collision by another vehicle, and the more severe side pole impact, protection was good all-round and the Defender scored maximum points for these tests. In an assessment of protection in far-side impact, dummy excursion (its movement towards the other side of the vehicle) was rated as adequate. The Defender is not equipped with a counter-measure to prevent occupant to occupant contact in side impacts. Tests on the front seats and head restraints demonstrated good protection against whiplash injury in the event of a rear-end collision. A geometric assessment of the rear seats also indicated good whiplash protection. The Defender has a multi-collision braking system which prevents secondary impacts. The car also has an advanced e-Call system which, in the event of an accident, automatically sends a message to the emergency services, giving the car's location.

In both the frontal offset test and the side barrier impact, protection of both child dummies was good for all critical parts of the body and the Defender scored maximum points in this part of the assessment. The front passenger airbag can be disabled to allow a rearward-facing child restraint to be used in that seating position. Clear information is provided to the driver regarding the status of the airbag and the system was rewarded. The Britax Römer KidFix XP (as a universal CRS, using the adult seatbelts) failed Euro NCAP's belt-length test for rearward-facing restraints in the optional third row seats. Used forward-facing, as Land Rover intend, the CRS can be properly installed. Land Rover state that a child restraint should not be installed in the optional front row 'jump seat'.

The bonnet provided predominantly good or adequate protection to the head of a struck pedestrian. The bumper provided good protection to pedestrians' legs. However, tests on the front edge of the bonnet revealed poor protection to a pedestrian’s pelvis at nearly all points across the width of the car. The Defender’s autonomous emergency braking (AEB) system can detect vulnerable road users like pedestrians and cyclists, as well as other vehicles. In tests, the system's response to pedestrians was adequate and to cyclists was good, with collisions avoided or mitigated in most cases. The system does not detect pedestrians to the rear of the car, and reversing tests were not performed.

Autonomous emergency braking (AEB) is fitted as standard. The system performed well in tests of its detection and reaction to other vehicles, with impacts being avoided or mitigated in most cases. The Defender has a seatbelt reminder for the front and rear seats, including the optional third row seats. The vehicle also has ‘Driver Drowsiness Detection’, which uses steering inputs to detect when the driver is fatigued or otherwise impaired and issues a warning if that is the case. The lane support system gently corrects the steering of the car if it is drifting out of lane and also intervenes more aggressively in some critical situations. A speed assistance system uses a camera and a digital map to detect the local speed limit. The driver can choose to let the system limit the speed appropriately.